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Anti-SIRT7 Antibody (C-term) at 1:2000 dilution + H-4-II-E whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 45 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
Western blot analysis of SIRT7 (arrow) using SIRT7 Antibody (C-term) (Cat. #P33646). HEK293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the SIRT7 gene (Lane 2).
P33646 staining SIRT7 in human esophagus tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
Rabbit Polyclonal Antibody to SIRT7
-
货号：
P33646 -
别名：
NAD-dependent protein deacetylase sirtuin-7, 351-, Regulatory protein SIR2 homolog 7, SIR2-like protein 7, SIRT7, SIR2L7 -
应用：
WB,IHC -
反应种属：
Human, Mouse, Rat -
抗体类型：
Primary antibody -
Swissprot：
Q9NRC8 -
规格：
-
数量：
-+ -
说明书：
目录价¥1980
Rabbit Polyclonal Antibody to SIRT7
Description |
|---|
SIRT7 is a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. |
Specification |
|
|---|---|
| Aliases | NAD-dependent protein deacetylase sirtuin-7, 351-, Regulatory protein SIR2 homolog 7, SIR2-like protein 7, SIRT7, SIR2L7 |
| Entrez GeneID | 51547 |
| Swissprot | Q9NRC8 |
| WB Predicted band size | 44.9kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | This SIRT7 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 331-360 amino acids from the C-terminal region of human SIRT7. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,1%BSA and 50% glycerol.prepared by Saturated Ammonium Sulfate (SAS) . |
Application |
|
|---|---|
| WB | 1/1000-1/2000 |
| IHC | 1/100-1/500 |
Product Image
- Anti-SIRT7 Antibody (C-term) at 1:2000 dilution + H-4-II-E whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 45 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
- Western blot analysis of SIRT7 (arrow) using SIRT7 Antibody (C-term) (Cat. #P33646). HEK293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the SIRT7 gene (Lane 2).
- P33646 staining SIRT7 in human esophagus tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
For Reseach Only
Application Key：WB - Western Blot | IHC - Immunohistochemistry | ICC - Immunocytochemistry | FCM - Flow Cytometry | ELISA - Enzyme-linked Immunosorbent Assay | IP - Immunoprecipitation
#P33646
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