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Western blot analysis of eIF4EBP1 in K562 lysates using 4E BP1 antibody.
Immunofluorescence analysis of 4E BP1 in HeLa using eIF4EBP1 antibody.
Immunohistochemistry analysis of paraffin-embedded Human breast carcinoma using eIF4EBP1 antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
Rabbit Polyclonal Antibody to 4E BP1
-
货号：
P22886 -
别名：
EIF4EBP1; Eukaryotic translation initiation factor 4E-binding protein 1; 4E-BP1; eIF4E-binding protein 1; Phosphorylated heat- and acid-stable protein regulated by insulin 1; PHAS-I -
应用：
WB,IP,IHC,IF,FCM -
反应种属：
Human,Mouse,Rat -
抗体类型：
Primary antibody -
Swissprot：
Q13541 -
规格：
-
数量：
-+ -
说明书：
目录价¥1980
Rabbit Polyclonal Antibody to 4E BP1
Description |
|---|
Translation repressor protein 4E-BP1 (also known as PHAS-1) inhibits cap-dependent translation by binding to the translation initiation factor eIF4E. Hyperphosphorylation of 4E-BP1 disrupts this interaction and results in activation of cap-dependent translation. Both the PI3 kinase/Akt pathway and FRAP/mTOR kinase regulate 4E-BP1 activity. |
Specification |
|
|---|---|
| Aliases | EIF4EBP1; Eukaryotic translation initiation factor 4E-binding protein 1; 4E-BP1; eIF4E-binding protein 1; Phosphorylated heat- and acid-stable protein regulated by insulin 1; PHAS-I |
| Entrez GeneID | 1978 |
| Swissprot | Q13541 |
| WB Predicted band size | Calculated MW: 13 kDa; Observed MW: 18 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human eIF4EBP1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
Application |
|
|---|---|
| WB | 1/500-1/1000 |
| IP | 1/10-1/20 |
| IHC | 1/50-1/100 |
| IF/ICC | 1/50-1/200 |
| FCM | 1/50-1/100 |
Product Image
- Western blot analysis of eIF4EBP1 in K562 lysates using 4E BP1 antibody.
- Immunofluorescence analysis of 4E BP1 in HeLa using eIF4EBP1 antibody.
- Immunohistochemistry analysis of paraffin-embedded Human breast carcinoma using eIF4EBP1 antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
For Reseach Only
Application Key：WB - Western Blot | IHC - Immunohistochemistry | ICC - Immunocytochemistry | FCM - Flow Cytometry | ELISA - Enzyme-linked Immunosorbent Assay | IP - Immunoprecipitation
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