Rabbit Monoclonal Antibody to Raf1

Description
Raf-1 is a MAP kinase kinase kinase (MAP3K) which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated Raf-1 can phosphorylate to activate the dual specificity protein kinases MEK1 and MEK2 which in turn phosphorylate to activate the serine/threonine specific protein kinases ERK1 and ERK2.

Specification
Aliases	RAF1; RAF; RAF proto-oncogene serine/threonine-protein kinase; Proto-oncogene c-RAF; cRaf; Raf-1
Entrez GeneID	5894
Swissprot	P04049
WB Predicted band size	Calculated MW: 73 kDa; Observed MW: 73 kDa
Host/Isotype	Rabbit IgG
Antibody Type	Primary antibody
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Human,Mouse,Rat
Immunogen	A synthetic peptide of human Raf1
Formulation	Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol.

Application
WB	1/500-1/1000
IF/ICC	1/50-1/200