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Western blot analysis of lysates from A431, HepG2, LNCaP cell line and mouse testis, rat testis tissue lysates (from left to right), using TPI1 Antibody (C-term) (Cat. #P32614). P32614 was diluted at 1:1000 at each lane.A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug per lane.
TPI1 Antibody (C-term) (Cat. #P32614) flow cytometric analysis of CEM cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Formalin-fixed and paraffin-embedded human prostate carcinoma reacted with TPI1 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Rabbit Polyclonal Antibody to TPI1
-
货号：
P32614 -
别名：
Triosephosphate isomerase, TIM, Triose-phosphate isomerase, TPI1, TPI -
应用：
WB,IHC,FCM -
反应种属：
Human, Mouse, Rat -
抗体类型：
Primary antibody -
Swissprot：
P60174 -
规格：
-
数量：
-+ -
说明书：
目录价¥1980
Rabbit Polyclonal Antibody to TPI1
Description |
|---|
TPI1 is an enzyme, consisting of two identical proteins, which catalyzes the isomerization of glyceraldehydes 3-phosphate (G3P) and dihydroxy-acetone phosphate (DHAP) in glycolysis and gluconeogenesis. |
Specification |
|
|---|---|
| Aliases | Triosephosphate isomerase, TIM, Triose-phosphate isomerase, TPI1, TPI |
| Entrez GeneID | 7167 |
| Swissprot | P60174 |
| WB Predicted band size | 26.7kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | This TPI1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 258-286 amino acids from the C-terminal region of human TPI1. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,1%BSA and 50% glycerol.prepared by Saturated Ammonium Sulfate (SAS) . |
Application |
|
|---|---|
| WB | 1/1000 |
| IHC | 1/100-1/500 |
| FCM | 1/10-1/50 |
Product Image
- Western blot analysis of lysates from A431, HepG2, LNCaP cell line and mouse testis, rat testis tissue lysates (from left to right), using TPI1 Antibody (C-term) (Cat. #P32614). P32614 was diluted at 1:1000 at each lane.A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug per lane.
- TPI1 Antibody (C-term) (Cat. #P32614) flow cytometric analysis of CEM cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
- Formalin-fixed and paraffin-embedded human prostate carcinoma reacted with TPI1 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
For Reseach Only
Application Key：WB - Western Blot | IHC - Immunohistochemistry | ICC - Immunocytochemistry | FCM - Flow Cytometry | ELISA - Enzyme-linked Immunosorbent Assay | IP - Immunoprecipitation
#P32614
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