Description

The protein encoded by the classic MBP gene is a major constituent of the myelin sheath of oligodendrocytes and Schwann cells in the nervous system. However, MBP-related transcripts are also present in the bone marrow and the immune system. These mRNAs arise from the long MBP gene (otherwise called "Golli-MBP") that contains 3 additional exons located upstream of the classic MBP exons. Alternative splicing from the Golli and the MBP transcription start sites gives rise to 2 sets of MBP-related transcripts and gene products. The Golli mRNAs contain 3 exons unique to Golli-MBP, spliced in-frame to 1 or more MBP exons. They encode hybrid proteins that have N-terminal Golli aa sequence linked to MBP aa sequence. The second family of transcripts contain only MBP exons and produce the well characterized myelin basic proteins. This complex gene structure is conserved among species suggesting that the MBP transcription unit is an integral part of the Golli transcription unit and that this arrangement is important for the function and/or regulation of these genes.

References
1. Cancer Epidemiol Biomarkers Prev. 2009 May;18(5):1651-8. 2. Biochemistry. 2009 Jun 9;48(22):4720-7.

Specification
Aliases	MGC99675
Entrez GeneID	4155
Swissprot	P02686
clone	2H9
WB Predicted band size	33kDa
Host/Isotype	Mouse IgG1
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Human
Immunogen	Purified recombinant fragment of human MBP expressed in E. Coli.
Formulation	Ascitic fluid containing 0.03% sodium azide.

Application
IHC	1/200 - 1/1000
ICC	1/200 - 1/1000
FCM	1/200 - 1/400
ELISA	1/10000