Description

The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein is a subunit of the protein complex that consists of MCM2-7. It has been shown to interact directly with MCM5/CDC46. This protein also interacts with and is acetylated by MCM3AP, a chromatin-associated acetyltransferase. The acetylation of this protein inhibits the initiation of DNA replication and cell cycle progression. Several transcript variants encoding different isoforms have been found for this gene.

References
1.Comput Math Methods Med. 2021 Oct 11;2021:8494260. 2.Biosci Rep. 2020 Jul 31;40(7):BSR20201503.

Specification
Aliases	HCC5; P1.h; RLFB; P1-MCM3
Entrez GeneID	4172
Swissprot	P25205
clone	1D4A3
WB Predicted band size	91kDa
Host/Isotype	Mouse IgG1
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Human, Monkey, Rat
Immunogen	Purified recombinant fragment of human MCM3 expressed in E. Coli.
Formulation	Purified antibody in PBS with 0.05% sodium azide

Application
WB	1/500 - 1/2000
IHC	1/200 - 1/1000
ICC	1/50 - 1/200
FCM	1/200 - 1/400